简介内容:Rhod-FF AM is a cell-permeant acetoxymethyl ester of the fluorescent calcium indicator rhod-FF . As rhod-FF AM enters cells, it is hydrolyzed by intracellular esterases to produce rhod-FF. Rhod-FF is a difluorinated analog of the cell-impermeant calcium indicator rhod-2. It has a very low affinity for calcium (Kd = 320 μM). Low affinity calcium indicators are particularly useful for studying compartments with high concentrations of calcium, such as endoplasmic reticulum, where high affinity dyes