简介内容:DiSBAC10, a voltage-sensitive fluorescent probe, facilitates cell membrane electrical activity investigation in FRET assays. At rest, it populates the membrane´s outer leaflet, accepting photons from excited fluorescein-labeled proteins and emitting them at higher wavelengths. Upon cell depolarization, DiSBAC10 quickly translocates to the inner leaflet, increasing fluorophore separation and diminishing the FRET signal.